Spanish Ministry of Economy and Competitiveness
The present project relies on previous experience of group members, and deals with the application in a coordinated fashion of different methodologies to study the biogenesis and assembly of membrane proteins. We aim to dig into this stepwise process by identifying new and existent components with the capacity to assist the targeting and integration of TM segments, by unveiling the mechanistic principles of TM insertion by the translocon, and by uncovering sequence patterns representing helix-helix packing motifs. Furthermore, we will apply the knowledge gained in our lab to investigate the putative role of TM segment insertion in a crucial biological process, i.e. Bcl2-dependent apoptosis.